Application

Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.

General description

PANSORBIN™ Cells are heat-killed, formalin fixed Staphylococcus aureus cells that have a coat of protein A and have been pickled by the method of Kessler. Useful for mitogenic stimulation of B lymphocytes and for immunoprecipitation.

Heat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high-affinity interaction of protein A with the Fc domain of IgG. PANSORBIN™ cells work best when the antibody is human (IgG₁, IgG₂, IgG₄), rabbit IgG (all isotypes), or mouse (IgG_2a, IgG_2b, IgG₃).

Legal Information

PANSORBIN is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kierszenbaum, F., et al. 1991. Immunology74, 317.Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.Murakami, H., et al. 1988. Biochem. J.256, 917.Kessler, S.W. 1975. J. Immunol. 115, 1617.

Packaging

25, 50 ml in Plastic ampoule

Physical form

Supplied as a ≥10% (w/v) Staphylococcus aureus cell suspension in PBS, 0.1% sodium azide, pH 7.2.

Warning

Toxicity: Standard Handling (A)